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Saudi Pharm J. 2016 May;24(3):241-4. doi: 10.1016/j.jsps.2016.04.005. Epub 2016 Apr 24.

Separation and purification and in vitro anti-proliferative activity of leukemia cell K562 of Galium aparine L. petroleum ether phase.

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School of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, PR China.
National Engineering Laboratory for Further Processing of Wheat and Corn, Henan University of Technology, Zhengzhou 450000, PR China.
School of Chemical Engineering and Energy, Zhengzhou University, Zhengzhou 450052, PR China.
Criminal Technique Institute of Public Security Bureau, Zhumadian, Henan, PR China.


To explore material basis of in vitro anti-proliferative activity of leukemia cell K562 of petroleum ether phase of product resulting from Galium aparine L. 60% ethanol extraction, the experiment adopts column chromatography combined with thin layer preparation, isolates and purifies petroleum ether, conducts structural identification of obtained single compound and applies MTT method for viability assay of in vitro anti-proliferative activity of leukemia cell K562. Experimental results show that G. aparine L. petroleum ether contains mainly β-sitosterol, daucosterol and dibutyl phthalate and other substances. Under experimental conditions, the three could inhibit the proliferation of leukemia cell K562 with dose-effect and time-effect relationship, of which dibutyl phthalate has strongest activity. Dibutyl phthalate with excellent activity, β-sitosterol with rich content and moderate effect should be the main contributor to its biological activity.


Galium aparine L.; K562; MTT; Purification

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