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Sci Rep. 2016 Jun 8;6:27636. doi: 10.1038/srep27636.

Increased airway glucose increases airway bacterial load in hyperglycaemia.

Author information

1
Mucosal Infection &Immunity Group, Section of Virology, Imperial College London, St Mary's Campus, London, W2 1PG, UK.
2
MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Imperial College London, London, SW7 2AZ, UK.
3
Airway Disease Infection Section, National Heart &Lung Institute, Imperial College London, London, W2 1PG, UK.
4
Institute for Infection and Immunity, St George's, University of London, London SW17 0RE, UK.
5
Health Protection Research Unit in Healthcare Associated Infection and Antimicrobial Resistance, Imperial College London, Hammersmith Campus, London W12 0HS, UK.

Abstract

Diabetes is associated with increased frequency of hospitalization due to bacterial lung infection. We hypothesize that increased airway glucose caused by hyperglycaemia leads to increased bacterial loads. In critical care patients, we observed that respiratory tract bacterial colonisation is significantly more likely when blood glucose is high. We engineered mutants in genes affecting glucose uptake and metabolism (oprB, gltK, gtrS and glk) in Pseudomonas aeruginosa, strain PAO1. These mutants displayed attenuated growth in minimal medium supplemented with glucose as the sole carbon source. The effect of glucose on growth in vivo was tested using streptozocin-induced, hyperglycaemic mice, which have significantly greater airway glucose. Bacterial burden in hyperglycaemic animals was greater than control animals when infected with wild type but not mutant PAO1. Metformin pre-treatment of hyperglycaemic animals reduced both airway glucose and bacterial load. These data support airway glucose as a critical determinant of increased bacterial load during diabetes.

PMID:
27273266
PMCID:
PMC4897689
DOI:
10.1038/srep27636
[Indexed for MEDLINE]
Free PMC Article

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