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Anticancer Res. 2016 Jun;36(6):2719-28.

Anticancer and Immunomodulatory Effects of Lactobacillus plantarum LS/07, Inulin and Melatonin in NMU-induced Rat Model of Breast Cancer.

Author information

1
Institute of Biology and Ecology, Faculty of Science, P.J. Šafárik University, Košice, Slovak Republic monika.kassayova@upjs.sk kruzliakpeter@gmail.com.
2
Department of Forensic Medicine, Faculty of Medicine, P.J. Šafárik University, Košice, Slovak Republic.
3
Department of Experimental Medicine, Faculty of Medicine, P.J. Šafárik University, Košice, Slovak Republic.
4
Institute of Biology and Ecology, Faculty of Science, P.J. Šafárik University, Košice, Slovak Republic.
5
Department of Medical Biology, Jessenius Faculty of Medicine, Comenius University in Bratislava, Martin, Slovak Republic.
6
Laboratory of Structural Biology and Proteomics, Central Laboratories, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic monika.kassayova@upjs.sk kruzliakpeter@gmail.com.
7
Department of Histology and Embryology, Jessenius Faculty of Medicine, Comenius University in Bratislava, Martin, Slovak Republic.

Abstract

BACKGROUND/AIM:

Chemopreventive activity of a new probiotic strain Lactobacillus plantarum LS/07 (PRO) and prebiotic oligofructose-enriched inulin (PRE) in rat mammary carcinogenesis induced by procarcinogen 7,12-dimethylbenz[a]anthracene has been reported before. This study evaluated the anticancer and immunomodulatory efficacy of PRO, PRE, PRO+PRE (PRO/PRE) and combination with melatonin (PRO+PRE+MEL) in a rat model, when breast cancer was induced by a direct-acting carcinogen N-nitroso-N-methylurea (NMU).

MATERIALS AND METHODS:

Daily administration of PRO (at a dose of 8.4×10(8) colony-forming units (c.f.u.)/rat), PRE (in the diet, 20 g/kg) and MEL (in tap water, 20 mg/l) started 14 days before the first NMU dose and lasted for 16 weeks.

RESULTS:

Although tumor growth was not altered, a marked decrease in the ratio of high-/low-grade carcinomas and in tumoral Ki-67 expression was found after PRO+PRE treatment; melatonin augmented these effects. PRO+PRE+MEL combination enhanced CD4(+) and CD8(+) T-cell tumor infiltration induced by PRO/PRE and increased CD25(+)FoxP3(+) regulatory T-cells in tumors.

CONCLUSION:

In mammary carcinogenesis, Lactobacillus plantarum LS/07 and inulin exert prodifferentiating, antiproliferative and immunomodulatory activities, which are significantly amplified by melatonin co-administration.

KEYWORDS:

Breast cancer; Lactobacillus plantarum; N-nitroso-N-methylurea; melatonin; oligofructose-enriched inulin; prebiotic; probiotic

PMID:
27272781
[Indexed for MEDLINE]

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