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J Exp Med. 2016 Jun 27;213(7):1117-31. doi: 10.1084/jem.20151885. Epub 2016 Jun 6.

Live cell imaging to understand monocyte, macrophage, and dendritic cell function in atherosclerosis.

Author information

1
Division of Inflammation Biology and Microscopy Core, La Jolla Institute of Allergy and Immunology, La Jolla, CA 92037.
2
Division of Inflammation Biology and Microscopy Core, La Jolla Institute of Allergy and Immunology, La Jolla, CA 92037 klaus@lji.org.

Abstract

Intravital imaging is an invaluable tool for understanding the function of cells in healthy and diseased tissues. It provides a window into dynamic processes that cannot be studied by other techniques. This review will cover the benefits and limitations of various techniques for labeling and imaging myeloid cells, with a special focus on imaging cells in atherosclerotic arteries. Although intravital imaging is a powerful tool for understanding cell function, it alone does not provide a complete picture of the cell. Other techniques, such as flow cytometry and transcriptomics, must be combined with intravital imaging to fully understand a cell's phenotype, lineage, and function.

PMID:
27270892
PMCID:
PMC4925021
DOI:
10.1084/jem.20151885
[Indexed for MEDLINE]
Free PMC Article

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