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ACS Appl Mater Interfaces. 2016 Jul 13;8(27):17047-59. doi: 10.1021/acsami.6b02936. Epub 2016 Jun 30.

A Fluorescent Polymer Probe with High Selectivity toward Vascular Endothelial Cells for and beyond Noninvasive Two-Photon Intravital Imaging of Brain Vasculature.

Author information

1
Laboratoire de Chimie, ENS de Lyon, CNRS UMR 5182, Université Claude Bernard, Université de Lyon , F69342, Lyon, France.
2
Grenoble Institut des Neurosciences, GIN, Inserm, U1216, Univ Grenoble Alpes , F-38000 Grenoble, France.
3
Advanced Materials Engineering and Modelling Group, Faculty of Chemistry, Wroclaw University of Technology , Wyb. Wyspianskiego 27, 50-370 Wroclaw, Poland.
4
CLINATEC, INSERM UA 01, Rue des Martyrs 17, 38054, Grenoble, France.

Abstract

A chromophore-engineering strategy that relies on the introduction of a ground-state distortion in a quadrupolar chromophore was used to obtain a quasi-quadrupolar chromophore with red emission and large two-photon absorption (2PA) cross-section in polar solvents. This molecule was functionalized with water-solubilizing polymer chains. It constitutes not only a remarkable contrast agent for intravital two-photon microscopy of the functional cerebral vasculature in a minimally invasive configuration but presents intriguing endothelial staining ability that makes it a valuable probe for premortem histological staining.

KEYWORDS:

TD-DFT; fluorescence; intravital microscopy; near infrared; star-shaped polymer; two-photon absorption

PMID:
27267494
DOI:
10.1021/acsami.6b02936

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