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Bull Exp Biol Med. 2016 May;161(1):50-3. doi: 10.1007/s10517-016-3342-1. Epub 2016 Jun 6.

Complex Approach to Xenobiotics Hepatotoxicity Testing using a Microfluidic System.

Author information

1
BioClinicum Research and Development Center, Moscow, Russia. a.aleksandrova@bioclinicum.com.
2
Moscow State University of Mechanical Engineering (MAMI), Moscow, Russia.
3
BioClinicum Research and Development Center, Moscow, Russia.

Abstract

We analyzed hepatotoxicity of three drugs: acetaminophen, metformin, and isoniazid. Spheroids of differentiated HepaRG cells cultured under microfluidic conditions were used as the model. Acute toxicity of substances was assessed by analyzing cell viability, while lactate concentration in the culture medium was used as the potential marker for evaluation of chronic exposure and non-lethal side effects of xenobiotics. The results were compared with mitochondrial activity and DNA fragmentation data. The efficiency and possibility of applying the integrated approach for assessment of drug hepatotoxicity are discussed.

KEYWORDS:

HepaRG; hepatotoxicity; xenobiotics

PMID:
27265135
DOI:
10.1007/s10517-016-3342-1
[Indexed for MEDLINE]

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