Format

Send to

Choose Destination
Bull Exp Biol Med. 2016 May;161(1):120-4. doi: 10.1007/s10517-016-3360-z. Epub 2016 Jun 6.

Maintenance of High Cytochrome P450 Expression in HepaRG Cell Spheroids in DMSO-Free Medium.

Author information

1
BioClinicum Research and Production Center, Moscow, Russia. a.aleksandrova@bioclinicum.com.
2
BioClinicum Research and Production Center, Moscow, Russia.
3
National Medical Research Radiology Center, Moscow, Russia.
4
Moscow State University of Mechanical Engineering (MAMI), Moscow, Russia.

Abstract

We studied the effects of DMSO and fibroblasts during HepaRG cell spheroid formation and conditions of their subsequent culturing on the levels of mRNA of the major cytochromes P450. A protocol of spheroid formation from differentiated HepaRG cells and their culturing in serum- and DMSO-free medium is developed.

KEYWORDS:

HepaRG; cytochromes P450; differentiation

PMID:
27265124
DOI:
10.1007/s10517-016-3360-z
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center