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Biochem Biophys Res Commun. 2016 Aug 5;476(4):607-613. doi: 10.1016/j.bbrc.2016.06.006. Epub 2016 Jun 3.

miR-411-5p inhibits proliferation and metastasis of breast cancer cell via targeting GRB2.

Author information

1
Department of Gastrointestinal Surgery, First Affiliated Hospital of Xiamen University, Xiamen 361003, China; State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen 361102, China.
2
Department of Gastrointestinal Surgery, First Affiliated Hospital of Xiamen University, Xiamen 361003, China; Department of Gastrointestinal Surgery, First Clinical Medical College of Fujian Medical University, Fuzhou 350005, China.
3
Department of Gastrointestinal Surgery, First Affiliated Hospital of Xiamen University, Xiamen 361003, China.
4
State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen 361102, China.
5
Department of Gastrointestinal Surgery, First Affiliated Hospital of Xiamen University, Xiamen 361003, China; Department of Gastrointestinal Surgery, First Clinical Medical College of Fujian Medical University, Fuzhou 350005, China. Electronic address: huangzhengjie@xmu.edu.cn.
6
Department of Gastrointestinal Surgery, First Affiliated Hospital of Xiamen University, Xiamen 361003, China; Department of Gastrointestinal Surgery, First Clinical Medical College of Fujian Medical University, Fuzhou 350005, China. Electronic address: luoqixmzsh@126.com.

Abstract

miR-411-5p (previously called miR-411) is severely involved in human diseases, however, the relationship between miR-411-5p and breast cancer has not been investigated thoroughly. Here, we found that the expression of miR-411-5p was downregulated in breast cancer tissues compared with their matched adjacent non-neoplastic tissues. In addition, the expression of miR-411-5p was also lower in breast cancer cell lines in contrast with MCF-10A. Moreover, we investigated the target and mechanism of miR-411-5p in breast cancer using mimic and inhibitor, and demonstrated the involvement of GRB2 and Ras activation. Ectopic expression of miR-411-5p suppressed the breast cancer cell proliferation, migration and invasion while low expression of miR-411-5p exhibited the opposite effect. Furthermore, GRB2 was demonstrated to be significantly overexpressed in breast cancer tissues compared with normal tissues, and low expression of GRB2 had a longer overall survival compared with high expression of GRB2 in breast cancer. In general, our study shed light on the miR-411-5p related mechanism in the progression of breast cancer and, miR-411-5p/GRB2/Ras axis is potential to be molecular target for breast cancer therapy.

KEYWORDS:

Breast cancer; GRB2; Metastasis; Proliferation; miR-411-5p

PMID:
27264952
DOI:
10.1016/j.bbrc.2016.06.006
[Indexed for MEDLINE]

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