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Arch Biochem Biophys. 2016 Aug 15;604:20-6. doi: 10.1016/j.abb.2016.06.001. Epub 2016 Jun 3.

IL-1β/NF-kb signaling promotes colorectal cancer cell growth through miR-181a/PTEN axis.

Author information

1
Department of Gastrointestinal Surgery, Xiangya Hospital Central South University, Changsha, Hunan, PR China.
2
Department of Oncology, Xiangya Hospital, Central-south University, Changsha, Hunan 410008, PR China.
3
Department of Oncology, Xiangya Hospital, Central-south University, Changsha, Hunan 410008, PR China. Electronic address: zhuhong0719@hotmail.com.

Abstract

To date, the role of miRNA in tumorigenesis has been largely reported. It was found that miR-181a may be involved in the tumorigenesis of colon cancer. The purpose of this study was to investigate the mechanism of miR-181a in colon cancer carcinogenesis. The expression levels of IL-1β, NF-κB (RelA), and miR-181a in colon cancer tissue were higher than that in normal control tissue when assessed by real-timePCR. In addition, it was found that IL-1β induced the expression of miR-181a. The expression of PTEN was regulated by IL-1β-stimulated miR-181a expression. In a PTEN reporter plasmid, miR-181a binding site mutations were introduced. By using a luciferase reporter assay, it was found that wild type reported activity was lower than that of the mutant registration system activity. Furthermore, a siRNA strategy was used to find that IL-1B regulates miR-181a expression via NF-κB and then regulates PTEN expression. Consequently, repression of PTEN by miR-181a promotes colon cancer cell proliferation. Taken together, our data support a critical role for NF-κB-dependent upregulation of miR-181a; this represents a new pathway for the repression of PTEN and the promotion of cell proliferation upon IL-1β induction.

KEYWORDS:

Colorectal caner; IL-1β; NF-κB; PTEN; miR-181a

PMID:
27264420
DOI:
10.1016/j.abb.2016.06.001
[Indexed for MEDLINE]
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