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Nanomedicine. 2016 Oct;12(7):2127-2137. doi: 10.1016/j.nano.2016.05.015. Epub 2016 Jun 1.

Response of heterogeneous cancer cells on targeted nanoparticles.

Author information

1
Guangdong Provincial Key laboratory of cancer immunotherapy Research, Guangzhou Key Laboratory of Tumor Immunology Research, Cancer Research Institute, School of Basic Medical Sciences, Southern Medical University. Electronic address: ningmengquan@gmail.com.
2
Department of Ultrasonography, Nanfang Hospital, Southern Medical University.
3
Guangdong Provincial Key laboratory of cancer immunotherapy Research, Guangzhou Key Laboratory of Tumor Immunology Research, Cancer Research Institute, School of Basic Medical Sciences, Southern Medical University; Department of Ultrasonography, Nanfang Hospital, Southern Medical University.
4
Guangdong Provincial Key laboratory of cancer immunotherapy Research, Guangzhou Key Laboratory of Tumor Immunology Research, Cancer Research Institute, School of Basic Medical Sciences, Southern Medical University.
5
School of Pharmaceutical Science, Southern Medical University.
6
Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.

Abstract

Heterogenous cancer cells possess cancer multidrug resistance (MDR) due to their relative quiescence and ABC-transporter expression. Heterogenous cancer cells can be detected by an Rh123 exclusion assay for identifying Rh123low population. In the present study, we fabricated targeted nanoparticles entrapped with Rh123 (Rh123 NPs) to investigate the effect of these targeted nanoparticles on an Rh123low population. The Rh123low population stained by Rh123 NPs exhibited similar heterogeneity to that stained by Rh123. In addition, the ABC-transporters did not contribute to the uptake of Rh123 or Rh123 NPs. Interestingly, ABC-transporters in the Rh123low population stained by Rh123 were possibly responsible for Rh123 efflux, while Rh123 NPs were not susceptible to ABC-transporters in the Rh123low population. It is plausible that the synergistic effect of NPs caused a targeted and endocytic effect which promoted the cellular uptake of Rh123 NPs, and the targeted effect played a more important role.

KEYWORDS:

Heterogenous cancer cells; MDR; Rh123; Targeted nanoparticles

PMID:
27262932
DOI:
10.1016/j.nano.2016.05.015
[Indexed for MEDLINE]

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