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Carbohydr Polym. 2016 Sep 20;149:112-20. doi: 10.1016/j.carbpol.2016.04.093. Epub 2016 Apr 26.

Exopolysaccharide from Trichoderma pseudokoningii induces macrophage activation.

Author information

1
Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241002, China.
2
Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241002, China.
3
Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
4
Xiangya Hospital, Central South University, Changsha, Hunan 410008, China. Electronic address: lhx900@aliyun.com.
5
Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241002, China; School of Life Science and National Glycoengineering Research Center, Shandong University, Jinan 250100, China. Electronic address: ksc313@126.com.

Abstract

In this study, we evaluated the immunomodulatory activity of an exopolysaccharide (EPS) derived from Trichoderma pseudokoningii and investigated the molecular mechanism of EPS-mediated activation of macrophages. Results revealed that EPS could significantly induce the production of nitric oxide (NO), tumor necrosis factor (TNF)-α and interleukin (IL)-1β and enhance phagocytic activity in RAW 264.7 cells. Immunofluorescence staining indicated that EPS promoted the nuclear translocation of nuclear factor (NF)-κB p65 subunit. Western blot analysis showed that EPS increased the expression of inducible nitric oxide synthase (iNOS) protein, the degradation of IκB-α and the phosphorylation of mitogen-activated protein kinases (MAPKs). Furthermore, pretreatment of RAW 264.7 cells with specific inhibitors of NF-κB and MAPKs significantly attenuated EPS-induced TNF-α and IL-1β production. EPS also induced the inhibition of cytokine secretion by special antibodies against Toll-like receptor-4 (TLR4) and Dectin-1. These data suggest that EPS from Trichoderma pseudokoningii activates RAW 264.7 cells through NF-κB and MAPKs signaling pathways via TLR4 and Dectin-1.

KEYWORDS:

Dimethyl sulfoxide (PubChem CID: 679); Exopolysaccharide; Immunomodulation; Lipopolysaccharide (PubChem CID: 11970143); MAPKs; Macrophages; NF-κB; Nitric oxide (PubChem CID: 145068); Trichoderma pseudokoningii

PMID:
27261736
DOI:
10.1016/j.carbpol.2016.04.093
[Indexed for MEDLINE]

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