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Anal Biochem. 2017 Sep 1;532:95-99. doi: 10.1016/j.ab.2016.05.014. Epub 2016 Jun 1.

Genome-scale CRISPR pooled screens.

Author information

1
New York Genome Center, New York, NY 10013, USA; Center for Genomics and Systems Biology, Department of Biology, New York University, New York, NY 10012, USA. Electronic address: nsanjana@nygenome.org.

Abstract

Genome editing technologies such as clustered regularly interspaced short palindromic repeats (CRISPR) systems have ushered in a new era of targeted DNA manipulation. The easy programmability of CRISPR using short oligonucleotides enables rapid synthesis of large-scale libraries for functional genetic screens. Here we present fundamental concepts and methods for pooled CRISPR screens and review biological results from recent genome-scale loss-of-function and gain-of-function screens. We also discuss new frontiers in pooled screens, including novel effector domains for functional screens and applications in the noncoding genome.

KEYWORDS:

CRISPR; Cancer; Cas9; Genetic screens; Genome engineering; Noncoding

PMID:
27261176
PMCID:
PMC5133192
DOI:
10.1016/j.ab.2016.05.014
[Indexed for MEDLINE]
Free PMC Article

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