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J Trace Elem Med Biol. 2016 Jul;36:1-6. doi: 10.1016/j.jtemb.2016.03.006. Epub 2016 Mar 14.

Oleuropein ameliorates arsenic induced oxidative stress in mice.

Author information

1
Department of Biochemistry, Faculty of Veterinary Medicine, Kafkas University, 36100 Kars, Turkey. Electronic address: metinogun@hotmail.com.
2
Department of Biochemistry, Faculty of Medicine, Kafkas University, 36100 Kars, Turkey.
3
Department of Pathology, Faculty of Veterinary Medicine, Balıkesir University, 36100 Balıkesir, Turkey.
4
Department of Biochemistry, Faculty of Veterinary Medicine, Kafkas University, 36100 Kars, Turkey.
5
Department of Pathology, Faculty of Veterinary Medicine, Kafkas University, 36100 Kars, Turkey.

Abstract

The objective of this study is to investigate the potential preventive effect of oleuropein in an experimental arsenic toxicity in mice. For this purpose, mice were exposed to 5mg/kg/day sodium arsenite (NaAsO2) in drinking water and treated with 30mg/kg/day oleuropein for 15 days. At the end of the experiment, animals were sacrificed and selected organs were processed for biochemical and histopahtological investigations. Blood, liver, kidney and brain malondialdehyde (MDA) and nitric oxide (NO) levels were determined by colorimetric methods. Protein carbonyl content is measured by a commercial kit. Liver morphology and immunoreactivity for inducible NOS (iNOS) and endothelial NOS (eNOS) was evaluated microscopically. Level of NO was determined to decrease in blood and tissues whereas MDA increased in arsenic given mice. Tissue protein carbonyl content also increased in this group. Immunoreactivity for iNOS and eNOS was noted to increase with arsenic treatment. Oleuropein treatment had significant effects in normalizing the MDA and NO levels as well as protein carbonyl content. Immunohistochemical staining also showed reduction of the expression of iNOS and eNOS in liver. The results indicate that oleuropein ameliorates oxidative tissue damage by scavenging free radicals.

KEYWORDS:

Arsenic toxicity; MDA; NO; Oleuropein; Oxidative damage; Protein carbonyl content

PMID:
27259345
DOI:
10.1016/j.jtemb.2016.03.006
[Indexed for MEDLINE]

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