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J Biomed Mater Res A. 2016 Nov;104(11):2649-57. doi: 10.1002/jbm.a.35803. Epub 2016 Jun 14.

Pro-osteoclastic in vitro effect of Polyethylene-like nanoparticles: Involvement in the pathogenesis of implant aseptic loosening.

Author information

1
Laboratory of Pathophysiology of Bone Resorption and Therapy of Primary Bone Tumours, INSERM, UMR 957, Nantes, 44035, France.
2
University of Nantes, Nantes Atlantique Universities, Nantes, France.
3
Nantes University Hospital, Nantes, France.
4
Laboratory of Pathophysiology of Bone Resorption and Therapy of Primary Bone Tumours, INSERM, UMR 957, Nantes, 44035, France. lcordova@stanford.edu.
5
University of Nantes, Nantes Atlantique Universities, Nantes, France. lcordova@stanford.edu.
6
Department of Oral and Maxillofacial Surgery-Faculty of Dentistry, University of Chile-Conicyt, Santiago, Chile. lcordova@stanford.edu.
7
CEISAM-UMR CNRS 6230, University of Nantes, Nantes, France.
8
MicroPICell Platform, SFR Santé François Bonamy, INSERM, UMS 016-UMS CNRS 3556, Nantes, France.
9
Department of Oncology and Metabolism, University of Sheffield, The Medical School, Sheffield, England, United Kingdom.

Abstract

Polyethylene micro-sized wear particles released from orthopedic implants promote inflammation and osteolysis; however, less is known about the bioactivity of polyethylene nanosized wear particles released from the last generation of polymer-bearing surfaces. We aim to assess the internalization of fluorescent polyethylene-like nanoparticles by both human macrophages and osteoclasts and also, to determine their effects in osteoclastogenesis in vitro. Human macrophages and osteoclasts were incubated with several ratios of fluorescent polyethylene-like nanoparticles between 1 and 72 h, and 4 h, 2, 4, 6, and 9 days, respectively. The internalization of nanoparticles was quantified by flow cytometry and followed by both confocal and video time-lapse microscopy. Osteoclast differentiation and activity was semiquantified by tartrate-resistant acid phosphatase (TRAP) staining, TRAP mRNA relative expression, and pit resorption assay, respectively. Macrophages, osteoclast precursors and mature osteoclasts internalized nanoparticles in a dose- and time-dependent manner and maintained their resorptive activity. In addition, nanoparticles significantly increased the osteoclastogenesis as shown by upregulation of the TRAP expressing cell number. We conclude that polyethylene-like nanosized wear particles promote osteoclast differentiation without alteration of bone resorptive activity of mature osteoclasts and they could be considered as important actors in periprosthetic osteolysis of the last new generation of polymer-bearing surfaces.

KEYWORDS:

aseptic loosening; macrophages; nanoparticles; osteoclasts; polyethylene

PMID:
27254768
DOI:
10.1002/jbm.a.35803
[Indexed for MEDLINE]
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