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Nat Plants. 2015 Jul 6;1:15094. doi: 10.1038/nplants.2015.94.

V-ATPase activity in the TGN/EE is required for exocytosis and recycling in Arabidopsis.

Author information

1
Department of Plant Systems Biology, VIB, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
2
Developmental Biology of Plants, Centre for Organismal Studies (COS), Heidelberg University, 69120 Heidelberg, Germany.
3
Max-Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany.
4
Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, 78000 Versailles, France.
5
AgroParisTech,Institut Jean-Pierre Bourgin, 78000 Versailles, France.
6
Department of Organic Chemistry, Polymer Chemistry Research Group and Laboratory for Organic Synthesis, Ghent University, 9000 Gent, Belgium.
7
Institute of Science and Technology Austria (IST Austria), 3400 Klosterneuburg, Austria.
8
Center for Plant Molecular Biology (ZMBP), University of Tübingen, 72076 Tübingen, Germany.
9
Australian Research Council, Centre of Excellence in Plant Cell Walls, School of Botany, University of Melbourne, Parkville, Victoria 3010, Australia.
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Contributed equally

Abstract

In plants, vacuolar H(+)-ATPase (V-ATPase) activity acidifies both the trans-Golgi network/early endosome (TGN/EE) and the vacuole. This dual V-ATPase function has impeded our understanding of how the pH homeostasis within the plant TGN/EE controls exo- and endocytosis. Here, we show that the weak V-ATPase mutant deetiolated3 (det3) displayed a pH increase in the TGN/EE, but not in the vacuole, strongly impairing secretion and recycling of the brassinosteroid receptor and the cellulose synthase complexes to the plasma membrane, in contrast to mutants lacking tonoplast-localized V-ATPase activity only. The brassinosteroid insensitivity and the cellulose deficiency defects in det3 were tightly correlated with reduced Golgi and TGN/EE motility. Thus, our results provide strong evidence that acidification of the TGN/EE, but not of the vacuole, is indispensable for functional secretion and recycling in plants.

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