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Molecules. 2016 May 30;21(6). pii: E711. doi: 10.3390/molecules21060711.

Molecular Genetic Characterization of an Anthrabenzoxocinones Gene Cluster in Streptomyces Sp. FJS31-2 for the Biosynthesis of BE-24566B and Zunyimycin Ale.

Author information

1
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China. 18585737637@163.com.
2
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China. changwuyue@hotmail.com.
3
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China. meiyun216@126.com.
4
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China. qianshengyan@126.com.
5
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. fussliu@126.com.
6
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China. baoyuxin8680915@126.com.
7
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China. wangmiao_good@163.com.
8
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. lysf1987313@163.com.
9
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China. xiaoqianli0908@163.com.
10
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China. yinyinwang0530@126.com.
11
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. huangy@im.ac.cn.

Abstract

Genome mining is an effective tool used to discover novel natural products from actinomycetes. Genome sequence analysis of Streptomyces sp. FJS31-2 revealed the presence of one putative type II polyketide gene cluster (ABX), which may correspond to type II polyketide products including BE-24566B and its chloro-derivatives. The addition of natural humus acid successfully activated the biosynthsis of the abx gene cluster. BE-24566B and its chloro-derivatives, named zunyimycin A, were also detected. The targeted deletion of the polyketide skeleton synthesis genes such as abxp, abxk, and abxs was performed in the wild strain to identify the gene cluster for BE-24566B biosynthesis.

KEYWORDS:

anthrabenzoxocinones; gene cluster; genetic engineering; genome mining; polyketide synthases

PMID:
27248985
PMCID:
PMC6273070
DOI:
10.3390/molecules21060711
[Indexed for MEDLINE]
Free PMC Article

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