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Autophagy. 2016 Aug 2;12(8):1411-2. doi: 10.1080/15548627.2016.1190891. Epub 2016 Jun 1.

Global analysis of cellular protein flux quantifies the selectivity of basal autophagy.

Author information

1
a Department of Biology , University of Rochester , Rochester , NY USA.

Abstract

In eukaryotic cells, the macroautophagy pathway has been implicated in the degradation of long-lived proteins and damaged organelles. Although it has been demonstrated that macroautophagy can selectively degrade specific targets, its contribution to the basal turnover of cellular proteins had previously not been quantified on proteome-wide scales. In a recent study, we utilized dynamic proteomics to provide a global comparison of protein half-lives between wild-type and autophagy-deficient cells. Our results indicated that in quiescent fibroblasts, macroautophagy contributes to the basal turnover of a substantial fraction of the proteome. However, the contribution of macroautophagy to constitutive protein turnover is variable within the proteome. The methodology outlined in the study provides a global strategy for quantifying the selectivity of basal macroautophagy.

KEYWORDS:

CCT/TRiC; basal autophagy; degradative flux; proteasome; proteomics; ribosome; selective autophagy; turnover

PMID:
27248575
PMCID:
PMC4968234
DOI:
10.1080/15548627.2016.1190891
[Indexed for MEDLINE]
Free PMC Article

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