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Islets. 2016 Apr 18;8(3):57-64. doi: 10.1080/19382014.2016.1161874.

Ginsenoside Rg3 prevents INS-1 cell death from intermittent high glucose stress.

Author information

1
a Division of Endocrinology and Metabolism, Department of Internal Medicine, Maryknoll Medical Center , Busan , South Korea.
2
b Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Inje University , Busan , South Korea.
3
c Paik Institute for Clinical Research, Molecular Therapy Lab, Inje University , Busan , South Korea.

Abstract

BACKGROUND:

Ginsenoside Rg3 has been proposed to mediate anti-diabetic effects, but their direct effect on pancreatic β cell viability and mechanisms are not clearly understood. Recent studies suggest that intermittent high glucose (IHG) could be more harmful to pancreatic β cells than sustained high glucose. There are few reports about the effect of the ginsenosideRg3 to β cell apoptosis and proliferation against IHG.

METHODS:

INS-1 cells were treated with alternative glucose concentration with or without ginsenoside Rg3. Cell apoptosis and viability were detected by Annexin V staining and MTT assay. The activation of mitogen-activated protein kinases (MAPKs) was analyzed by Western blotting using specific antibodies. Quantification of secreted insulin protein was measured using rat/mouse Insulin ELISA kits. Bromodeoxyuridine (BrdU) staining and florescence in situ hybridization (FISH) analysis was performed to compare cell proliferation.

RESULT:

INS-1 cell viability was decreased under IHG and increased with Rg3 treatment.Rg3 significantly reduced the apoptotic INS-1 cells against IHG. The quantification of secreted insulin concentration was increased with Rg3. Rg3 increased INS-1 cell proliferation. ERK and p38 MAPK pathways reduced by IHG were activated by the ginsenoside Rg3.

CONCLUSION:

Ginsenoside Rg3 protected INS-1 cell death from IHG with reducing apoptosis and increasing proliferation.

KEYWORDS:

anti-apoptosis; cell proliferation; cytoprotection; ginsenoside Rg3; insulin-secreting cells

PMID:
27246809
PMCID:
PMC4987017
DOI:
10.1080/19382014.2016.1161874
[Indexed for MEDLINE]
Free PMC Article

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