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Nanomedicine (Lond). 2016 Aug;11(15):1929-46. doi: 10.2217/nnm-2016-0150. Epub 2016 Jun 1.

Neuronal commitment of human circulating multipotent cells by carbon nanotube-polymer scaffolds and biomimetic peptides.

Author information

1
Department of Biology, University of Padua, 35131 Padua, Italy.
2
Department of Pharmaceutical & Pharmacological Sciences, University of Padua, 35131 Padua, Italy.
3
Department of Chemical Sciences, University of Padua, 35131 Padua, Italy.
4
Department of Woman & Child Health, University of Padua, 35128 Padua, Italy.
5
Tissue Engineering & Signaling ONLUS, Caselle di Selvazzano Dentro, 35030 Padua, Italy.

Abstract

AIM:

We aimed to set up a self-standing, biomimetic scaffold system able to induce and support per se neuronal differentiation of autologous multipotent cells.

MATERIALS & METHODS:

We isolated a population of human circulating multipotent cells (hCMCs), and used carbon nanotube/polymer nanocomposite scaffolds to mimic electrical/nanotopographical features of the neural environment, and biomimetic peptides reproducing axon guidance cues from neural proteins.

RESULTS:

hCMCs showed high degree of stemness and multidifferentiative potential; stimuli from the scaffolds and biomimetic peptides could induce and boost hCMC differentiation toward neuronal lineage despite the absence of exogenously added, specific growth factors.

CONCLUSION:

This work suggests the scaffold-peptides system combined with autologous hCMCs as a functional biomimetic, self-standing prototype for neural regenerative medicine applications.

KEYWORDS:

biomimetic peptides; carbon nanotubes; human circulating multipotent cells; nanocomposite scaffold; neuronal differentiation; regenerative medicine

PMID:
27246559
DOI:
10.2217/nnm-2016-0150
[Indexed for MEDLINE]

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