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Plant Physiol. 2017 Jan;173(1):240-255. doi: 10.1104/pp.16.00095. Epub 2016 May 31.

Defective Pollen Wall 2 (DPW2) Encodes an Acyl Transferase Required for Rice Pollen Development.

Xu D1,2,3,4,5,6,7,8, Shi J1,2,3,4,5,6,7,8, Rautengarten C1,2,3,4,5,6,7,8, Yang L1,2,3,4,5,6,7,8, Qian X1,2,3,4,5,6,7,8, Uzair M1,2,3,4,5,6,7,8, Zhu L1,2,3,4,5,6,7,8, Luo Q1,2,3,4,5,6,7,8, An G1,2,3,4,5,6,7,8, Waßmann F1,2,3,4,5,6,7,8, Schreiber L1,2,3,4,5,6,7,8, Heazlewood JL1,2,3,4,5,6,7,8, Scheller HV1,2,3,4,5,6,7,8, Hu J1,2,3,4,5,6,7,8, Zhang D1,2,3,4,5,6,7,8, Liang W9,10,11,12,13,14,15,16.

Author information

1
Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (D.X., J.S., L.Y., X.Q., M.U., L.Z., Q.L., D.Z., W.L.).
2
ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia (C.R., J.L.H.).
3
Joint BioEnergy Institute and Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (C.R., J.L.H., H.V.S.).
4
Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea (G.A.).
5
Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (F.W., L.S.).
6
Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 (H.V.S.).
7
Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (J.H.); and.
8
School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
9
Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (D.X., J.S., L.Y., X.Q., M.U., L.Z., Q.L., D.Z., W.L.); wqliang@sjtu.edu.cn.
10
ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia (C.R., J.L.H.); wqliang@sjtu.edu.cn.
11
Joint BioEnergy Institute and Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (C.R., J.L.H., H.V.S.); wqliang@sjtu.edu.cn.
12
Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea (G.A.); wqliang@sjtu.edu.cn.
13
Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (F.W., L.S.); wqliang@sjtu.edu.cn.
14
Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 (H.V.S.); wqliang@sjtu.edu.cn.
15
Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (J.H.); and wqliang@sjtu.edu.cn.
16
School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.) wqliang@sjtu.edu.cn.

Abstract

Aliphatic and aromatic lipids are both essential structural components of the plant cuticle, an important interface between the plant and environment. Although cross links between aromatic and aliphatic or other moieties are known to be associated with the formation of leaf cutin and root and seed suberin, the contribution of aromatic lipids to the biosynthesis of anther cuticles and pollen walls remains elusive. In this study, we characterized the rice (Oryza sativa) male sterile mutant, defective pollen wall 2 (dpw2), which showed an abnormal anther cuticle, a defective pollen wall, and complete male sterility. Compared with the wild type, dpw2 anthers have increased amounts of cutin and waxes and decreased levels of lipidic and phenolic compounds. DPW2 encodes a cytoplasmically localized BAHD acyltransferase. In vitro assays demonstrated that recombinant DPW2 specifically transfers hydroxycinnamic acid moieties, using ω-hydroxy fatty acids as acyl acceptors and hydroxycinnamoyl-CoAs as acyl donors. Thus, The cytoplasmic hydroxycinnamoyl-CoA:ω-hydroxy fatty acid transferase DPW2 plays a fundamental role in male reproduction via the biosynthesis of key components of the anther cuticle and pollen wall.

PMID:
27246096
PMCID:
PMC5210703
DOI:
10.1104/pp.16.00095
[Indexed for MEDLINE]
Free PMC Article

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