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Methods Mol Biol. 2016;1422:309-27. doi: 10.1007/978-1-4939-3603-8_27.

Characterization of Colorectal Cancer Development in Apc (min/+) Mice.

Author information

1
Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, 660 S. Euclid Ave, Box 8118, St. Louis, MO, 63110, USA. INalbantoglu@path.wustl.edu.
2
Department of Medicine, Washington University School of Medicine in St. Louis, St. Louis, MO, USA.

Abstract

The Apc (min/+) mouse provides an excellent experimental model for studying genetic, environmental, and therapeutic aspects of intestinal neoplasia in humans. In this chapter, we will describe techniques for studying colon cancer development in Apc (min/+) mice on C57BL/6J (B6) background, focusing on the roles of environmental modifiers, including Dextran Sulfate Sodium (DSS), high fat diet, and bile acid supplementation in the context of experimental colorectal cancer. This chapter also includes protocols describing extraction and purification of DSS-contaminated RNA, as well as sampling, harvesting, and tissue processing. The common pathologic lesions encountered in these animals are described in detail.

KEYWORDS:

Adenoma; Apc min/+; Bile acids; Colorectal cancer; Dextran sulfate sodium; High fat

PMID:
27246043
PMCID:
PMC5061497
DOI:
10.1007/978-1-4939-3603-8_27
[Indexed for MEDLINE]
Free PMC Article

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