Format

Send to

Choose Destination
Methods Mol Biol. 2016;1422:49-61. doi: 10.1007/978-1-4939-3603-8_6.

Ussing Chamber Technique to Measure Intestinal Epithelial Permeability.

Author information

1
Department of Radiation Oncology, University of Florida Shands Cancer Center, Cancer and Genetics Research Complex, 2033 Mowry Road, Box 103633, Gainesville, FL, 32610, USA. vidyasagar@ufl.edu.
2
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT, 06520-8026, USA.

Abstract

Epithelial cells are polarized and have tight junctions that contribute to barrier function. Assessment of barrier function typically involves measurement of electrophysiological parameters or movement of nonionic particles across an epithelium. Here, we describe measurement of transepithelial electrical conductance or resistance, determination of dilution potential, and assessment of flux of nonionic particles such as dextran or mannitol, with particular emphasis on Ussing chamber techniques.

KEYWORDS:

Diffusion potential; Paracellular permeability; Tight junction; Transepithelial resistance

PMID:
27246022
DOI:
10.1007/978-1-4939-3603-8_6
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center