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Methods Mol Biol. 2016;1412:191-203. doi: 10.1007/978-1-4939-3539-0_20.

Assay of Endocannabinoid Uptake.

Author information

1
Institute of Biochemistry and Molecular Medicine, NCCR TransCure, University of Bern, Bern, Switzerland.
2
Institute of Biochemistry and Molecular Medicine, NCCR TransCure, University of Bern, Bern, Switzerland. gertsch@ibmm.unibe.ch.

Abstract

Endocannabinoids at physiological concentrations are crossing cellular membranes by facilitated diffusion, a process that can be studied by measuring transport kinetics. Here, we describe a radiosubstrate-based assay using arachidonoyl[1-(3)H]ethanolamine or arachidonoyl[1,2,3-(3)H]glycerol to measure the cellular endocannabinoid uptake in a three-phase assay with human U937 cells.

KEYWORDS:

2-Arachidonoylglycerol; Anandamide; Endocannabinoid uptake

PMID:
27245905
DOI:
10.1007/978-1-4939-3539-0_20
[Indexed for MEDLINE]

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