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J Food Sci. 2016 Jul;81(7):C1646-53. doi: 10.1111/1750-3841.13352. Epub 2016 May 31.

Chemical Profiling Using Uplc Q-Tof/Ms and Antioxidant Activities of Fortunella Fruits.

Tan S1,2, Zhao X1,2, Yang Y1,2, Ke Z1,2, Zhou Z1,2.

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College of Horticulture and Landscape Architecture, Southwest Univ, Chongqing, 400716, China.
Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing, 400715, China.


The fruits of Fortunella Swingle are widely consumed as fresh fruits and traditional medicine in China. China is the origin center and has the largest cultivated area of the genus Fortunella. In this study, the chemical compositions of ethanol extracts of the major Fortunella cultivated types including Fortunella japonica Swingle, Fortunella margarita Swingle, Fortunella crassifolia Swingle 1 (Lanshang) and Fortunella crassifolia Swingle 2 (Liuyang) were determined using ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC Q-TOF/MS) method, and their antioxidant activities were evaluated. 12 compounds were identified and 5 compounds were tentatively characterized. The results showed that the chemical compositions of the ethanol extracts of 4 Fortunella cultivated types were largely the same. 3', 5'-di-C-glucopyranosylphloretin was the predominant flavonoid in Fortunella fruits, and Fortunella margarita Swingle had higher contents of flavonoids than other species. In addition, the data demonstrated high antioxidant activities of Fortunella fruits. The developed method could be available to rapidly analyze the chemical compounds in Fortunella fruits and its products. This study will provide information for further quality assessment and utilization of Fortunella resources.


Fortunella fruits; UPLC Q-TOF/MS; antioxidant capacity; chemical compositions

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