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Genomics. 2016 Aug;108(2):56-63. doi: 10.1016/j.ygeno.2016.05.004. Epub 2016 May 27.

Natural antisense transcription from a comparative perspective.

Author information

1
RNA Interest Group, Institute for Cell and Molecular Biosciences, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom.
2
RNA Interest Group, Institute for Cell and Molecular Biosciences, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom. Electronic address: Andreas.werner@ncl.ac.uk.

Abstract

Natural antisense transcripts (NATs) can interfere with the expression of complementary sense transcripts with exquisite specificity. We have previously cloned NATs of Slc34a loci (encoding Na-phosphate transporters) from fish and mouse. Here we report the cloning of a human SLC34A1-related NAT that represents an alternatively spliced PFN3 transcript (Profilin3). The transcript is predominantly expressed in testis. Phylogenetic comparison suggests two distinct mechanisms producing Slc34a-related NATs: Alternative splicing of a transcript from a protein coding downstream gene (Pfn3, human/mouse) and transcription from the bi-directional promoter (Rbpja, zebrafish). Expression analysis suggested independent regulation of the complementary Slc34a mRNAs. Analysis of randomly selected bi-directionally transcribed human/mouse loci revealed limited phylogenetic conservation and independent regulation of NATs. They were reduced on X chromosomes and clustered in regions that escape inactivation. Locus structure and expression pattern suggest a NATs-associated regulatory mechanisms in testis unrelated to the physiological role of the sense transcript encoded protein.

KEYWORDS:

Evolution; Human; Mouse; Na-phosphate transport; Natural antisense transcript

PMID:
27241791
PMCID:
PMC4996343
DOI:
10.1016/j.ygeno.2016.05.004
[Indexed for MEDLINE]
Free PMC Article

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