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Curr Opin Chem Biol. 2016 Oct;34:20-29. doi: 10.1016/j.cbpa.2016.05.011. Epub 2016 May 27.

From dirt to industrial applications: Pseudomonas putida as a Synthetic Biology chassis for hosting harsh biochemical reactions.

Author information

1
Systems and Synthetic Biology Program, Centro Nacional de Biotecnología (CNB-CSIC), 28049 Madrid, Spain. Electronic address: pablo.nikel@cnb.csic.es.
2
Escuela de Química & CIPRONA, Universidad de Costa Rica, 11501-2060 San José, Costa Rica.
3
AMAbiotics SAS, Institut of Cardiometabolism and Nutrition (ICAN), Hôpital Universitaire de la Pitié-Salpêtrière, 75013 Paris, France.
4
Systems and Synthetic Biology Program, Centro Nacional de Biotecnología (CNB-CSIC), 28049 Madrid, Spain. Electronic address: vdlorenzo@cnb.csic.es.

Abstract

The soil bacterium Pseudomonas putida is endowed with a central carbon metabolic network capable of fulfilling high demands of reducing power. This situation arises from a unique metabolic architecture that encompasses the partial recycling of triose phosphates to hexose phosphates-the so-called EDEMP cycle. In this article, the value of P. putida as a bacterial chassis of choice for contemporary, industrially-oriented metabolic engineering is addressed. The biochemical properties that make this bacterium adequate for hosting biotransformations involving redox reactions as well as toxic compounds and intermediates are discussed. Finally, novel developments and open questions in the continuous quest for an optimal microbial cell factory are presented at the light of current and future needs in the area of biocatalysis.

PMID:
27239751
DOI:
10.1016/j.cbpa.2016.05.011
[Indexed for MEDLINE]
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