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Fish Shellfish Immunol. 2016 Aug;55:257-66. doi: 10.1016/j.fsi.2016.05.037. Epub 2016 May 27.

First characterization of three cyclophilin family proteins in the oyster, Crassostrea ariakensis Gould.

Author information

1
Laboratory of Marine Life Science and Technology, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, China; School of Life Sciences, Shaoxing University, Shaoxing, Zhejiang, China.
2
Laboratory of Marine Life Science and Technology, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
3
School of Life Sciences, Shaoxing University, Shaoxing, Zhejiang, China.
4
Laboratory of Marine Life Science and Technology, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, China; Ocean College, Qinzhou University, Qinzhou City, Guangxi, China. Electronic address: wuxz@zju.edu.cn.

Abstract

Cyclophilins (CyPs) are a family of proteins that bind the immunosuppressive agent cyclosporin A (CsA) with high-affinity and belong to one of the three superfamilies of peptidyl-prolyl cis-trans isomerases (PPIase). In this report, three cyclophilin genes (Ca-CyPs), including Ca-CyPA, Ca-CyPB and Ca-PPIL3, were identified from oyster, Crassostrea ariakensis Gould in which Ca-CyPA encodes a protein with 165 amino acid sequences, Ca-CyPB encodes a protein with 217 amino acid sequences and Ca-PPIL3 encodes a protein with 162 amino acid sequences. All of the three Ca-CyPs genes contain a typical CyP-PPIase domain with its signature sequences and Ca-CyPB contains an N-signal peptide sequences. Tissue distribution study revealed that Ca-CyPs were ubiquitously expressed in all examined tissues and the highest levels were observed in hemocytes. RLO incubation upregulated the mRNA expression levels of Ca-CyPs, indicating that three Ca-CyPs might be involved in oyster immune response against RLO infection.

KEYWORDS:

Ca-CyPs; Crassostrea ariakensis; Cyclophilin; Immune response; Rickettsia-like organism

PMID:
27238430
DOI:
10.1016/j.fsi.2016.05.037
[Indexed for MEDLINE]

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