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Stem Cell Reports. 2016 Jun 14;6(6):825-833. doi: 10.1016/j.stemcr.2016.04.015. Epub 2016 May 26.

H1foo Has a Pivotal Role in Qualifying Induced Pluripotent Stem Cells.

Author information

1
Department of Cardiology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan.
2
Department of Cardiology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan. Electronic address: yuasa@keio.jp.
3
Laboratory Animal Resource Center, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan.
4
Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
5
Department of Pediatrics, Keio University School of Medicine, Tokyo 160-8582, Japan.
6
Division of Pulmonary, Critical Care and Sleep Medicine, Departments of Medicine and Genetics and Genomic Sciences, Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
7
Sakaguchi Laboratory, Department of Systems Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan.
8
Electron Microscope Laboratory, Keio University School of Medicine, Tokyo 160-8582, Japan.
9
Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo 160-8582, Japan.

Abstract

Embryonic stem cells (ESCs) are a hallmark of ideal pluripotent stem cells. Epigenetic reprogramming of induced pluripotent stem cells (iPSCs) has not been fully accomplished. iPSC generation is similar to somatic cell nuclear transfer (SCNT) in oocytes, and this procedure can be used to generate ESCs (SCNT-ESCs), which suggests the contribution of oocyte-specific constituents. Here, we show that the mammalian oocyte-specific linker histone H1foo has beneficial effects on iPSC generation. Induction of H1foo with Oct4, Sox2, and Klf4 significantly enhanced the efficiency of iPSC generation. H1foo promoted in vitro differentiation characteristics with low heterogeneity in iPSCs. H1foo enhanced the generation of germline-competent chimeric mice from iPSCs in a manner similar to that for ESCs. These findings indicate that H1foo contributes to the generation of higher-quality iPSCs.

PMID:
27237376
PMCID:
PMC4912480
DOI:
10.1016/j.stemcr.2016.04.015
[Indexed for MEDLINE]
Free PMC Article

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