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Methods Mol Biol. 2016;1428:231-43. doi: 10.1007/978-1-4939-3625-0_15.

Delivery of Synthetic mRNA Encoding FOXP3 Antigen into Dendritic Cells for Inflammatory Breast Cancer Immunotherapy.

Author information

1
Division of Surgical Sciences, Department of Surgery, Women's Cancer Program, Duke Cancer Institute, Duke University School of Medicine, Durham, NC, 27710, USA. gayathri.devi@duke.edu.
2
Division of Surgical Sciences, Department of Surgery, Women's Cancer Program, Duke Cancer Institute, Duke University School of Medicine, Durham, NC, 27710, USA.

Abstract

Dendritic cell (DC)-based vaccines are commonly used for cancer immunotherapy. To prepare vaccines, DCs are pulsed or transfected with either: (a) defined peptides of tumor-associated antigens, (b) total protein isolated from the tumor cell, (c) autologous total RNA isolated from the tumor cell, (d) synthetic tumor-antigen-encoding mRNA, or (e) genes that encode for specific tumor-associated antigens. Introduction of tumor-associated antigen(s) and subsequent generation of mature DCs that can stimulate tumor-antigen-specific cytotoxic T lymphocytes comprise the critical steps of cancer vaccine preparation. Here, we described a method of: (a) preparing and delivering synthetic FOXP3 mRNA into human DCs, (b) generating mature DCs.

KEYWORDS:

Cancer vaccines; Cytotoxic T lymphocyte (CTL) assay; Dendritic cell; Electroporation; Lipofection; Synthetic mRNA; Tumor-associated antigens

PMID:
27236803
DOI:
10.1007/978-1-4939-3625-0_15
[Indexed for MEDLINE]

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