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Meat Sci. 2016 Nov;121:27-32. doi: 10.1016/j.meatsci.2016.05.007. Epub 2016 May 10.

A gene and protein expression study on four porcine genes related to intramuscular fat deposition.

Author information

1
Department of Agricultural and Food Sciences (DISTAL), University of Bologna, Viale Fanin 46, 40127 Bologna, Italy. Electronic address: martina.zappaterra2@unibo.it.
2
Center for Applied Biomedical Research (C.R.B.A.), S. Orsola-Malpighi University Hospital, University of Bologna, Via Massarenti 9, 40138 Bologna, Italy; Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Via Massarenti 9, 40138 Bologna, Italy. Electronic address: marzia.deserti@gmail.com.
3
Center for Applied Biomedical Research and Endocrinology Unit, Department of Medical and Surgical Sciences, University of Bologna, Via Massarenti 9, 40138 Bologna, Italy. Electronic address: mazza.roberta@gmail.com.
4
Department of Agricultural and Food Sciences (DISTAL), University of Bologna, Viale Fanin 46, 40127 Bologna, Italy. Electronic address: silvia_braglia@yahoo.it.
5
Department of Agricultural and Food Sciences (DISTAL), University of Bologna, Viale Fanin 46, 40127 Bologna, Italy. Electronic address: paolo.zambonelli@unibo.it.
6
Department of Agricultural and Food Sciences (DISTAL), University of Bologna, Viale Fanin 46, 40127 Bologna, Italy. Electronic address: roberta.davoli@unibo.it.

Abstract

Intramuscular fat (IMF) content has a prominent role in meat quality, affecting sensory attributes such as flavour and texture. In the present research, we studied in samples of porcine Semimembranosus muscle four genes related to lipid metabolism and whose gene expressions have been associated to IMF deposition: FASN, SCD, LIPE and LPL. We analysed both mRNA and protein expressions in two groups of Italian Large White pigs divergent for Semimembranosus IMF deposition, with the aim of comparing the levels of four genes and enzymes between the two groups and identifying possible coexpression links. The obtained results suggest a prominent role of LIPE enzyme in IMF hydrolysis, as the samples with low IMF deposition show a significantly higher amount of this lipase. Finally, a poorly known correlation was found between LIPE and FASN enzymes only in female individuals. These results provide new information for the understanding of IMF deposition.

KEYWORDS:

Adipogenesis; FASN; Fat genes; Gene expression; LIPE; LPL; Lipid metabolism; Meat quality; Muscle fat; Pig; Protein; SCD

PMID:
27236338
DOI:
10.1016/j.meatsci.2016.05.007
[Indexed for MEDLINE]

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