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Dev Biol. 2016 Nov 1;419(1):19-25. doi: 10.1016/j.ydbio.2016.05.029. Epub 2016 May 26.

Multi-sample Arabidopsis Growth and Imaging Chamber (MAGIC) for long term imaging in the ZEISS Lightsheet Z.1.

Author information

  • 1Plant and Microbial Biology Department, North Carolina State University, Raleigh, USA.
  • 2Plant and Microbial Biology Department, North Carolina State University, Raleigh, USA; Biological Engineering Department, North Carolina State University, Raleigh, USA.
  • 3Plant and Microbial Biology Department, North Carolina State University, Raleigh, USA; Physics and Biology Departments, North Carolina State University, Raleigh, USA; Industrial and Systems Engineering Department, North Carolina State University, Raleigh, USA.
  • 4Cellular and Molecular Imaging Facility, North Carolina State University, Raleigh, USA.
  • 5Industrial and Systems Engineering Department, North Carolina State University, Raleigh, USA. Electronic address: tjhorn.ims@gmail.com.
  • 6Plant and Microbial Biology Department, North Carolina State University, Raleigh, USA. Electronic address: ross_sozzani@ncsu.edu.

Abstract

Time-course imaging experiments on live organisms are critical for understanding the dynamics of growth and development. Light-sheet microscopy has advanced the field of long-term imaging of live specimens by significantly reducing photo-toxicity and allowing fast acquisition of three-dimensional data over time. However, current light-sheet technology does not allow the imaging of multiple plant specimens in parallel. To achieve higher throughput, we have developed a Multi-sample Arabidopsis Growth and Imaging Chamber (MAGIC) that provides near-physiological imaging conditions and allows high-throughput time-course imaging experiments in the ZEISS Lightsheet Z.1. Here, we illustrate MAGIC's imaging capabilities by following cell divisions, as an indicator of plant growth and development, over prolonged time periods. To automatically quantify the number of cell divisions in long-term experiments, we present a FIJI-based image processing pipeline. We demonstrate that plants imaged with our chamber undergo cell divisions for >16 times longer than those with the glass capillary system supplied by the ZEISS Z1.

KEYWORDS:

3D printing; Arabidopsis root; Light sheet; Time-lapse imaging

PMID:
27235815
DOI:
10.1016/j.ydbio.2016.05.029
[PubMed - in process]
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