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J Sep Sci. 2016 Jul;39(14):2846-52. doi: 10.1002/jssc.201600005. Epub 2016 Jun 29.

Determination of hexabromocyclododecane enantiomers in chicken whole blood by a modified quick, easy, cheap, effective, rugged, and safe method with liquid chromatography and tandem mass spectrometry.

Author information

1
Key Laboratory of Applied Technology of Sophisticated Analytical Instruments, Shandong Academy of Sciences, Jinan, Shandong, China.
2
School of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan, Shandong, China.

Abstract

A rapid and simple analytical method has been developed for the determination of hexabromocyclododecane enantiomers in chicken whole blood, based on a modified quick, easy, cheap, effective, rugged, and safe approach before liquid chromatography coupled with tandem mass spectrometry. The factors influencing performance of method were investigated by single factor experiment, and further optimized by the response surface methodology based on Box-Behnken design. The matrix effects were also evaluated by the isotopic dilution method. Under the optimal conditions, the proposed method showed good linearity within the range of 1-500 μg/L and good repeatability with relative standard deviation less than 9.5% (n = 5). The limits of detection (S/N = 3) were 0.03-0.19 μg/L. The developed method was successfully applied for the analysis of hexabromocyclododecane enantiomers in real chicken blood samples. The satisfactory recoveries ranging of 83.6-115.0% were obtained (at spiked levels of 5, 20, and 100 μg/L). The results demonstrated that the proposed method would be a practical value method for the determination of hexabromocyclododecane enantiomers in animal blood. It would be further developed with confidence to analyze other lipophilic organic pollutants in blood sample.

KEYWORDS:

Chicken whole blood; Enantiomeric selectivity; Liquid chromatography with tandem mass spectrometry; QuEChERS

PMID:
27235093
DOI:
10.1002/jssc.201600005
[Indexed for MEDLINE]

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