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EJNMMI Res. 2016 Dec;6(1):44. doi: 10.1186/s13550-016-0199-2. Epub 2016 May 27.

Comparison of LDPI to SPECT perfusion imaging using (99m)Tc-sestamibi and (99m)Tc-pyrophosphate in a murine ischemic hind limb model of neovascularization.

Author information

1
Department of Nuclear Medicine, Maastricht University Medical Centre (MUMC+), Postbox 5800, AZ, 6202, Maastricht, The Netherlands.
2
Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, The Netherlands.
3
Department of Physiology, CARIM, Maastricht University, Maastricht, The Netherlands.
4
Department of Nuclear Medicine, Maastricht University Medical Centre (MUMC+), Postbox 5800, AZ, 6202, Maastricht, The Netherlands. felix.mottaghy@mumc.nl.
5
Department of Nuclear Medicine, University hospital, RWTH University, Aachen, Germany. felix.mottaghy@mumc.nl.

Abstract

BACKGROUND:

We aimed to determine the accuracy of laser Doppler perfusion imaging (LDPI) in an animal model for hind limb ischemia.

METHODS:

We used a murine (C57Bl/6 mice) ischemic hind limb model in which we compared LDPI with the clinically used (99m)Tc-sestamibi SPECT perfusion imaging (n = 7). In addition, we used the SPECT tracer (99m)Tc-pyrophosphate ((99m)Tc-PyP) to image muscular damage (n = 6).

RESULTS:

LDPI indicated a quick and prominent decrease in perfusion immediately after ligation, subsequently recovering to 21.9 and 25.2 % 14 days later in the (99m)Tc-sestamibi and (99m)Tc-PyP group, respectively. (99m)Tc-sestamibi SPECT scans also showed a quick decrease in perfusion. However, nearly full recovery was reached 7 days post ligation. Muscular damage, indicated by the uptake of (99m)Tc-PyP, was highest at day 3 and recovered to baseline levels at day 14 post ligation. Postmortem histology supported these findings, as a significantly increased collateral diameter was found 7 and 14 days after ligation and peak macrophage infiltration and TUNEL positivity was found on day 3 after ligation.

CONCLUSIONS:

Here, we indicate that LDPI strongly underestimates perfusion recovery in a hind limb model for profound ischemia.

KEYWORDS:

Hind limb ischemia; Laser Doppler perfusion imaging (LDPI); Overestimation of therapeutic window; Perfusion recovery; Single-photon emission computed tomography (SPECT)

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