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Mol Nutr Food Res. 2017 Jan;61(1). doi: 10.1002/mnfr.201501025. Epub 2016 Aug 11.

Obese ZDF rats fermented resistant starch with effects on gut microbiota but no reduction in abdominal fat.

Author information

1
Pennington Biomedical Research Center, Baton Rouge, LA, USA.
2
School of Nutrition and Food Sciences, Louisiana State University AgCenter, LA, USA.
3
Division of Pulmonary and Critical Care Medicine, Louisiana State University Health Sciences Center, New Orleans, LA, USA.
4
Department of Microbiology, Immunology and Parasitology, Louisiana State University Health Sciences Center, New Orleans, LA, USA.
5
Department of Pathobiological Sciences, Louisiana State University Veterinary School, LA, USA.
6
Tulane National Primate Center, Covington, LA, USA.
7
Ingredion Incorporated, Westchester, NJ, USA.
8
Western USDA Research Center, Albany, CA, USA.
9
Experimental Statistics, Louisiana State University, Baton Rouge, LA, USA.

Abstract

SCOPE:

To determine if whole-grain (WG) flour with resistant starch (RS) will produce greater fermentation than isolated RS in obese Zucker Diabetic Fatty (ZDF) rats, and whether greater fermentation results in different microbiota, reduced abdominal fat, and increased insulin sensitivity.

METHODS AND RESULTS:

This study utilized four groups fed diets made with either isolated digestible control starch, WG control flour (6.9% RS), isolated RS-rich corn starch (25% RS), or WG corn flour (25% RS). ZDF rats fermented RS and RS-rich WG flour to greatest extent among groups. High-RS groups had increased serum glucagon-like peptide 1 (GLP-1) active. Feeding isolated RS showed greater Bacteroidetes to Firmicutes phyla among groups, and rats consuming low RS diets possessed more bacteria in Lactobacillus genus. However, no differences in abdominal fat were observed, but rats with isolated RS had greatest insulin sensitivity among groups.

CONCLUSIONS:

Data demonstrated ZDF rats (i) possess a microbiota that fermented RS, and (ii) WG high-RS fermented better than purified RS. However, fermentation and microbiota changes did not translate into reduced abdominal fat. The defective leptin receptor may limit ZDF rats from responding to increased GLP-1 and different microbiota for reducing abdominal fat, but did not prevent improved insulin sensitivity.

KEYWORDS:

Fermentation; Leptin signaling; Microbiota; Obese ZDF rats; Resistant starch; Whole-grain

PMID:
27234399
PMCID:
PMC5324831
DOI:
10.1002/mnfr.201501025
[Indexed for MEDLINE]
Free PMC Article

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