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Enzyme Microb Technol. 2016 Jul;89:63-8. doi: 10.1016/j.enzmictec.2016.03.006. Epub 2016 Mar 18.

Improvement of ectoine productivity by using sugar transporter-overexpressing Halomonas elongata.

Author information

1
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai-cho, Nada, Kobe 657-8501, Japan.
2
Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai-cho, Nada, Kobe 657-8501, Japan.
3
Division of Environmental Protection Technology, Institute of Environmental Science, Graduate School of Fisheries and Environmental Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
4
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai-cho, Nada, Kobe 657-8501, Japan. Electronic address: akondo@kobe-u.ac.jp.

Abstract

We successfully enhanced the productivity of ectoine with Halomonas elongata by improvement of the transport of sugar. First, we carried out screening for sugar transporters capable of improving glucose and xylose consumption. We found two transporters: b3657 from Escherichia coli, which is capable of improving glucose consumption, and HEO_0208 from H. elongata, which is capable of improving xylose consumption. Using transporter-overexpressing strains, the productivity of ectoine was improved. These results indicate that sugar consumption is important for efficient ectoine production. As result of phenotypic analysis of a HEO_0208 deletion strain, we discovered that HEO_0208 is the major xylose transporter in H. elongata. This is the first report demonstrating improvement of ectoine productivity by enhancing the transport of sugar.

KEYWORDS:

Ectoine; Glucose; Halomonas elongata; Sugar transport; Xylose

PMID:
27233128
DOI:
10.1016/j.enzmictec.2016.03.006
[Indexed for MEDLINE]

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