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Plant J. 2016 Sep;87(6):629-40. doi: 10.1111/tpj.13224. Epub 2016 Aug 5.

Requirement for flap endonuclease 1 (FEN1) to maintain genomic stability and transcriptional gene silencing in Arabidopsis.

Author information

1
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
2
Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China.
3
Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN, 47906, USA.
4
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China. gongzz@cau.edu.cn.

Abstract

As a central component in the maturation of Okazaki fragments, flap endonuclease 1 (FEN1) removes the 5'-flap and maintains genomic stability. Here, FEN1 was cloned as a suppressor of transcriptional gene silencing (TGS) from a forward genetic screen. FEN1 is abundant in the root and shoot apical meristems and FEN1-GFP shows a nucleolus-localized signal in tobacco cells. The Arabidopsis fen1-1 mutant is hypersensitive to methyl methanesulfonate and shows reduced telomere length. Interestingly, genome-wide chromatin immunoprecipitation and RNA sequencing results demonstrate that FEN1 mutation leads to a decrease in the level of H3K27me3 and an increase in the expression of a subset of genes marked with H3K27me3. Overall, these results uncover a role for FEN1 in mediating TGS as well as maintaining genome stability in Arabidopsis.

KEYWORDS:

Arabidopsis thaliana; ChIP-seq; DNA damage repair; FEN1; H3K27me3; telomere; transcriptional gene silencing

PMID:
27231839
PMCID:
PMC5508578
DOI:
10.1111/tpj.13224
[Indexed for MEDLINE]
Free PMC Article

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