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Science. 2016 May 27;352(6289):1102-5. doi: 10.1126/science.aae0109.

Nuclear-localized cyclic nucleotide-gated channels mediate symbiotic calcium oscillations.

Author information

1
Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK. myriam.charpentier@jic.ac.uk giles.oldroyd@jic.ac.uk.
2
Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
3
Computational and Systems Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
4
Laboratoire de Biochimie et Physiologie Moléculaire des Plantes, UMR 5004 CNRS-386 INRA (French National Institute for Agricultural Research)-SupAgro-M-Université Montpellier, Campus SupAgro-INRA, 34060 Montpellier, France.
5
Metabolic Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.

Abstract

Nuclear-associated Ca(2+) oscillations mediate plant responses to beneficial microbial partners--namely, nitrogen-fixing rhizobial bacteria that colonize roots of legumes and arbuscular mycorrhizal fungi that colonize roots of the majority of plant species. A potassium-permeable channel is known to be required for symbiotic Ca(2+) oscillations, but the calcium channels themselves have been unknown until now. We show that three cyclic nucleotide-gated channels in Medicago truncatula are required for nuclear Ca(2+) oscillations and subsequent symbiotic responses. These cyclic nucleotide-gated channels are located at the nuclear envelope and are permeable to Ca(2+) We demonstrate that the cyclic nucleotide-gated channels form a complex with the postassium-permeable channel, which modulates nuclear Ca(2+) release. These channels, like their counterparts in animal cells, might regulate multiple nuclear Ca(2+) responses to developmental and environmental conditions.

PMID:
27230377
DOI:
10.1126/science.aae0109
[Indexed for MEDLINE]
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