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Bioanalysis. 2016 Jun;8(12):1237-50. doi: 10.4155/bio-2016-0013. Epub 2016 May 26.

Determination of ibuprofen enantiomers in human plasma by HPLC-MS/MS: validation and application in neonates.

Author information

1
Department of Pharmacy, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai 201102, China.
2
Central Laboratory, Shanghai Xuhui Central Hospital, Shanghai, China.
3
Department of Neonatology, Children's Hospital of Fudan University, Shanghai, China.

Abstract

AIM:

An adaptive method to determine ibuprofen enantiomers with limited volume of plasma required is necessary for investigating PK of ibuprofen in neonates.

RESULTS:

Enantiomer separation was achieved on a Lux cellulose 3 column with mobile phase consisting of methanol water (85:15, v/v) and formic acid (0.0075%) at isocratic rate of 0.2 ml/min. Calibration curve is linear for each enantiomer at the range of 0.1-60 μg/ml. Validation was conducted and results met requirements regarding to intra- and inter-run precision, accuracy and recovery. No matrix effect or interference was observed from neonatal plasma or comedications. Only 20 μl of plasma was requested in this study.

CONCLUSION:

This assay was specific and reliable to quantify ibuprofen enantiomers in neonate plasma.

KEYWORDS:

HPLC–MS/MS; enantiomers; ibuprofen; interference; neonate

PMID:
27225837
DOI:
10.4155/bio-2016-0013
[Indexed for MEDLINE]

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