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Chem Asian J. 2016 Jul 5;11(13):1892-5. doi: 10.1002/asia.201600626. Epub 2016 Jun 6.

DNA Triplexes-Guided Assembly of G-Quadruplexes for Constructing Label-free Fluorescent Logic Gates.

Author information

1
Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
2
University of Chinese Academy of Sciences, Beijing, 100049, China.
3
School of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, 450001, China.
4
School of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, 450001, China. zhangjy@zzu.edu.cn.
5
Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China. rjpei2011@sinano.ac.cn.

Abstract

Assembly of G-quadruplexes guided by DNA triplexes in a controlled manner is achieved for the first time. The folding of triplex sequences in acidic conditions brings two separated guanine-rich sequences together and subsequently a G-quadruplex structure is formed in the presence of K(+) . Based on this novel platform, label-free fluorescent logic gates, such as AND, INHIBIT, and NOR, are constructed with ions as input and the fluorescence of a G-quadruplex-specific fluorescent probe NMM as output.

KEYWORDS:

DNA; G-quadruplexes; fluorescent probes; self-assembly; triplex DNA

PMID:
27224871
DOI:
10.1002/asia.201600626
[Indexed for MEDLINE]

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