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Food Chem Toxicol. 2016 Aug;94:1-10. doi: 10.1016/j.fct.2016.05.009. Epub 2016 May 17.

Epicatechin-3-gallate reverses TGF-β1-induced epithelial-to-mesenchymal transition and inhibits cell invasion and protease activities in human lung cancer cells.

Author information

1
Department of Medicine Chest, Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan.
2
Medical Education Center, Kaohsiung Armed Forces General Hospital, Kaohsiung City, Taiwan; Institute of Biochemistry, Microbiology and Immunology, Chung Shang Medical University, Taichung City, Taiwan.
3
Institute of Biochemistry, Microbiology and Immunology, Chung Shang Medical University, Taichung City, Taiwan.
4
Institute and Department of Food Science, Central Taiwan University of Science and Technology, Taiwan.
5
Institute of Biochemistry, Microbiology and Immunology, Chung Shang Medical University, Taichung City, Taiwan; Clinical Laboratory, Chung Shan Medical University Hospital, No 110, Section 1, Jianguo N. Road, Taichung, Taiwan. Electronic address: peini@csmu.edu.tw.

Abstract

Epithelial-to-mesenchymal transition (EMT) and invasion potential have been considered as essential factors in cancer metastasis, which is the major cause of cancer death. EMT is a multi-step process that involves gain invasion, cytoskeleton change, cell adhesion, and proteolytic extracellular matrix degradation. Epicatechin-3-gallate (ECG), which is a natural polyphenolic component of green tea, elicits several antioxidant and anti-inflammatory effects. However, the effects of ECG on cancer invasion and EMT of human lung carcinoma remain unknown. We provided molecular evidence supporting the anti-metastatic effect of ECG. This compound suppressed the invasion (P < 0.001) of highly metastatic A549 cells by reducing the activities of matrix metalloproteinase-2 (P < 0.001) and urokinasetype plasminogen activator (P < 0.001). ECG also reversed the transforming growth factor (TGF)-β1-induced EMT and upregulated epithelial markers, such as E-cadherin. Conversely, ECG inhibited mesenchymal markers, such as fibronectin and p-FAK. The subcutaneous inoculation of this compound also inhibited the tumor growth of the A549 cells in vivo. Therefore, ECG may be used as an anti-cancer and anti-invasion agent for the adjuvant treatment and metastasis control of human lung cancer cells. ECG may also be administered as an effective chemopreventive agent against TGF-β1-induced EMT.

KEYWORDS:

ECG; EMT; Lung cancer; Metastasis; Polyphenol

PMID:
27224248
DOI:
10.1016/j.fct.2016.05.009
[Indexed for MEDLINE]

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