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Chem Biol Interact. 2017 Apr 1;267:104-115. doi: 10.1016/j.cbi.2016.05.028. Epub 2016 May 22.

Biotechnological applications of quorum quenching enzymes.

Author information

1
Aix Marseille Université, URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, Marseille, France.
2
Gene&GreenTK, Faculté de Médecine, 27 boulevard Jean Moulin, 13385 Marseille Cedex 5, France.
3
Aix Marseille Université, URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, Marseille, France; Gene&GreenTK, Faculté de Médecine, 27 boulevard Jean Moulin, 13385 Marseille Cedex 5, France.
4
University of Minnesota, Department of Biochemistry, Molecular Biology and Biophysics & Biotechnology Institute, St. Paul, MN 55108, USA.
5
Aix Marseille Université, URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, Marseille, France. Electronic address: eric.chabriere@univ-amu.fr.

Abstract

Numerous bacteria use quorum sensing (QS) to synchronize their behavior and monitor their population density. They use signaling molecules known as autoinducers (AI's) that are synthesized and secreted into their local environment to regulate QS-dependent gene expression. Among QS-regulated pathways, biofilm formation and virulence factor secretion are particularly problematic as they are involved in surface-attachment, antimicrobial agent resistance, toxicity, and pathogenicity. Targeting QS represents a promising strategy to inhibit undesirable bacterial traits. This strategy, referred to as quorum quenching (QQ), includes QS-inhibitors and QQ enzymes. These approaches are appealing because they do not directly challenge bacterial survival, and consequently selection pressure may be low, yielding a lower occurrence of resistance. QQ enzymes are particularly promising because they act extracellularly to degrade AI's and can be used in catalytic quantities. This review draws an overview of QQ enzyme related applications, covering several economically important fields such as agriculture, aquaculture, biofouling and health issues. Finally, the possibility of resistance mechanism occurrence to QQ strategies is discussed.

KEYWORDS:

Biofilm; Enzyme; Lactonase; Quorum quenching; Quorum sensing; Virulence

PMID:
27223408
DOI:
10.1016/j.cbi.2016.05.028
[Indexed for MEDLINE]

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