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Nat Methods. 2016 Jul;13(7):563-567. doi: 10.1038/nmeth.3871. Epub 2016 May 23.

Comparison of Cas9 activators in multiple species.

Author information

1
Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA.
2
Department of Pathology, Massachusetts General Hospital, Boston, Massachusetts, USA.
3
Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA.
4
Department of Molecular Biology and Biochemistry, Middlebury College, Middlebury, VT, USA.
5
Department of Mathematics, Middlebury College, Middlebury, VT, USA.
6
Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts, USA.
7
Institute for Medical Engineering & Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
8
Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
9
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
10
Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
#
Contributed equally

Abstract

Several programmable transcription factors exist based on the versatile Cas9 protein, yet their relative potency and effectiveness across various cell types and species remain unexplored. Here, we compare Cas9 activator systems and examine their ability to induce robust gene expression in several human, mouse, and fly cell lines. We also explore the potential for improved activation through the combination of the most potent activator systems, and we assess the role of cooperativity in maximizing gene expression.

PMID:
27214048
PMCID:
PMC4927356
DOI:
10.1038/nmeth.3871
[Indexed for MEDLINE]
Free PMC Article

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