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Stem Cells Int. 2016;2016:7235757. doi: 10.1155/2016/7235757. Epub 2016 Apr 26.

Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins.

Author information

1
Division of Regenerative Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka 540-0006, Japan.
2
Division of Stem Cell Research, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka 540-0006, Japan.
3
Division of Regenerative Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka 540-0006, Japan; Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
4
Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.
5
Department of Orthopedic Surgery, Keio University School of Medicine, Tokyo 160-8582, Japan.
6
Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
7
Division of Regenerative Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka 540-0006, Japan; Department of Neurosurgery, Osaka National Hospital, National Hospital Organization, Osaka 540-0006, Japan.

Abstract

Human neural progenitor cells (hNPCs) have previously been generated from limited numbers of human induced pluripotent stem cell (hiPSC) clones. Here, 21 hiPSC clones derived from human dermal fibroblasts, cord blood cells, and peripheral blood mononuclear cells were differentiated using two neural induction methods, an embryoid body (EB) formation-based method and an EB formation method using dual SMAD inhibitors (dSMADi). Our results showed that expandable hNPCs could be generated from hiPSC clones with diverse somatic tissue origins. The established hNPCs exhibited a mid/hindbrain-type neural identity and uniform expression of neural progenitor genes.

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