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Neural Regen Res. 2016 Apr;11(4):630-5. doi: 10.4103/1673-5374.180749.

A mouse model of weight-drop closed head injury: emphasis on cognitive and neurological deficiency.

Author information

1
Faculty of Medicine and Defence Health, National Defence University of Malaysia, Kem Sungai Besi, Kuala Lumpur, Malaysia.
2
Chemistry Department, Centre for Defence Foundation Studies, National Defence University of Malaysia, Kem Sungai Besi, Kuala Lumpur, Malaysia.
3
Department of Civil Engineering, Faculty of Engineering, National Defence University of Malaysia, Kem Sungai Besi, Kuala Lumpur, Malaysia.
4
Centre for Research and Innovation Management, National Defence University of Malaysia, Kem Sungai Besi, Kuala Lumpur, Malaysia.
5
Scientific Centre for Expertise of Medical Application Products, Petrovsky Blvd, Moscow, Russia.

Abstract

Traumatic brain injury (TBI) is a leading cause of death and disability in individuals worldwide. Producing a clinically relevant TBI model in small-sized animals remains fairly challenging. For good screening of potential therapeutics, which are effective in the treatment of TBI, animal models of TBI should be established and standardized. In this study, we established mouse models of closed head injury using the Shohami weight-drop method with some modifications concerning cognitive deficiency assessment and provided a detailed description of the severe TBI animal model. We found that 250 g falling weight from 2 cm height produced severe closed head injury in C57BL/6 male mice. Cognitive disorders in mice with severe closed head injury could be detected using passive avoidance test on day 7 after injury. Findings from this study indicate that weight-drop injury animal models are suitable for further screening of brain neuroprotectants and potentially are similar to those seen in human TBI.

KEYWORDS:

C57BL/6 mice; nerve regeneration; neural regeneration; neurological severity score; passive avoidance; traumatic brain injury; weight-drop injury model

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