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Cell Rep. 2016 May 31;15(9):2076-88. doi: 10.1016/j.celrep.2016.04.067. Epub 2016 May 19.

Leader Cells Define Directionality of Trunk, but Not Cranial, Neural Crest Cell Migration.

Author information

1
Randall Division of Cell & Molecular Biophysics, King's College, London SE1 1UL, UK.
2
Laboratory for Scientific Image Analysis (SCIAN-Lab), Biomedical Neuroscience Institute (BNI), Centre for Medical Informatics and Telemedicine (CIMT), ICBM, Faculty of Medicine, University of Chile, Santiago 1058, Chile.
3
Instituto de Biologia Molecular de Barcelona, CSIC, Parc Cientific de Barcelona, C/Baldiri i Reixac 15-21, Barcelona 08028, Spain.
4
ICBM Human Genetics Program, Centre for Medical Informatics and Telemedicine (CIMT), Faculty of Medicine, University of Chile, Santiago 1058, Chile.
5
Engineering Department, Cambridge University, Trumpington Street, Cambridge CB2 1PZ, UK.
6
Randall Division of Cell & Molecular Biophysics, King's College, London SE1 1UL, UK. Electronic address: claudia.linker@kcl.ac.uk.

Abstract

Collective cell migration is fundamental for life and a hallmark of cancer. Neural crest (NC) cells migrate collectively, but the mechanisms governing this process remain controversial. Previous analyses in Xenopus indicate that cranial NC (CNC) cells are a homogeneous population relying on cell-cell interactions for directional migration, while chick embryo analyses suggest a heterogeneous population with leader cells instructing directionality. Our data in chick and zebrafish embryos show that CNC cells do not require leader cells for migration and all cells present similar migratory capacities. In contrast, laser ablation of trunk NC (TNC) cells shows that leader cells direct movement and cell-cell contacts are required for migration. Moreover, leader and follower identities are acquired before the initiation of migration and remain fixed thereafter. Thus, two distinct mechanisms establish the directionality of CNC cells and TNC cells. This implies the existence of multiple molecular mechanisms for collective cell migration.

PMID:
27210753
PMCID:
PMC4893160
DOI:
10.1016/j.celrep.2016.04.067
[Indexed for MEDLINE]
Free PMC Article

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