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Prog Biophys Mol Biol. 2016 Jul;121(2):123-30. doi: 10.1016/j.pbiomolbio.2016.05.007. Epub 2016 May 19.

The potential impact of new generation transgenic methods on creating rabbit models of cardiac diseases.

Author information

1
Rabbit Genome and Biomodel Group, NARIC-Agricultural Biotechnology Institute, H-2100 Gödöllő, Hungary. Electronic address: bosze@abc.hu.
2
Rabbit Genome and Biomodel Group, NARIC-Agricultural Biotechnology Institute, H-2100 Gödöllő, Hungary.
3
Department of Pharmacology & Pharmacotherapy, University of Szeged, H-6720 Szeged, Hungary.
4
Department of Cardiology and Angiology I, University Heart Center Freiburg, D-79106 Freiburg, Germany.
5
Department of Pharmacology & Pharmacotherapy, University of Szeged, H-6720 Szeged, Hungary; Rabbit Genome and Biomodel Group, NARIC-Agricultural Biotechnology Institute, H-2100 Gödöllő, Hungary.

Abstract

Since the creation of the first transgenic rabbit thirty years ago, pronuclear microinjection remained the single applied method and resulted in numerous important rabbit models of human diseases, including cardiac deficiencies, albeit with low efficiency. For additive transgenesis a novel transposon mediated method, e.g., the Sleeping Beauty transgenesis, increased the efficiency, and its application to create cardiac disease models is expected in the near future. The targeted genome engineering nuclease family, e.g., the zink finger nuclease (ZFN), the transcription activator-like effector nuclease (TALEN) and the newest, clustered regularly interspaced short palindromic repeats (CRISPR) with the CRISPR associated effector protein (CAS), revolutionized the non-mouse transgenesis. The latest gene-targeting technology, the CRISPR/CAS system, was proven to be efficient in rabbit to create multi-gene knockout models. In the future, the number of tailor-made rabbit models produced with one of the above mentioned methods is expected to exponentially increase and to provide adequate models of heart diseases.

KEYWORDS:

Designer nucleases; Knock-in; Knockout; Sleeping beauty transposon; Transgenic rabbit

[Indexed for MEDLINE]

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