Format

Send to

Choose Destination
Plant Physiol. 2016 Jul;171(3):2069-84. doi: 10.1104/pp.15.01625. Epub 2016 May 20.

RNA-Seq Links the Transcription Factors AINTEGUMENTA and AINTEGUMENTA-LIKE6 to Cell Wall Remodeling and Plant Defense Pathways.

Author information

1
Department of Biological Sciences, University of South Carolina, Columbia, South Carolina (B.A.K., C.J.B., K.X., Z.Q.F., J.W.S.); andDepartment of Bioinformatics and Genomics, University of North Carolina, Charlotte, North Carolina (I.C.B., A.E.L.) krizek@sc.edu ann.loraine@uncc.edu.
2
Department of Biological Sciences, University of South Carolina, Columbia, South Carolina (B.A.K., C.J.B., K.X., Z.Q.F., J.W.S.); andDepartment of Bioinformatics and Genomics, University of North Carolina, Charlotte, North Carolina (I.C.B., A.E.L.).

Abstract

AINTEGUMENTA (ANT) and AINTEGUMENTA-LIKE6 (AIL6) are two related transcription factors in Arabidopsis (Arabidopsis thaliana) that have partially overlapping roles in several aspects of flower development, including floral organ initiation, identity specification, growth, and patterning. To better understand the biological processes regulated by these two transcription factors, we performed RNA sequencing (RNA-Seq) on ant ail6 double mutants. We identified thousands of genes that are differentially expressed in the double mutant compared with the wild type. Analyses of these genes suggest that ANT and AIL6 regulate floral organ initiation and growth through modifications to the cell wall polysaccharide pectin. We found reduced levels of demethylesterified homogalacturonan and altered patterns of auxin accumulation in early stages of ant ail6 flower development. The RNA-Seq experiment also revealed cross-regulation of AIL gene expression at the transcriptional level. The presence of a number of overrepresented Gene Ontology terms related to plant defense in the set of genes differentially expressed in ant ail6 suggest that ANT and AIL6 also regulate plant defense pathways. Furthermore, we found that ant ail6 plants have elevated levels of two defense hormones: salicylic acid and jasmonic acid, and show increased resistance to the bacterial pathogen Pseudomonas syringae These results suggest that ANT and AIL6 regulate biological pathways that are critical for both development and defense.

PMID:
27208279
PMCID:
PMC4936541
DOI:
10.1104/pp.15.01625
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center