Format

Send to

Choose Destination
Plant Physiol. 2016 Oct;172(2):668-689. Epub 2016 May 15.

Transcriptomes of Eight Arabidopsis thaliana Accessions Reveal Core Conserved, Genotype- and Organ-Specific Responses to Flooding Stress.

Author information

1
Plant Ecophysiology, Institute of Environmental Biology, Utrecht University, 3584 CH Utrecht, The Netherlands (H.v.V., D.V., E.R., S.H., M.K., J.B.-S., L.A.C.J.V., R.S.);Institute of Life Sciences, Scuola Superiore Sant'Anna, 56127 Pisa, Italy (H.v.V.);Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, 1090 GE Amsterdam, The Netherlands (M.A., P.v.T.);Center for Plant Cell Biology, Botany, and Plant Sciences, University of California, Riverside, California 92521 (T.G., J.B.-S.);Department of Plant Physiology, Bayreuth University, 95447 Bayreuth, Germany (A.M.); andBiosystematics Group, Wageningen University, 6708 PB Wageningen, The Netherlands (M.E.S.).
2
Plant Ecophysiology, Institute of Environmental Biology, Utrecht University, 3584 CH Utrecht, The Netherlands (H.v.V., D.V., E.R., S.H., M.K., J.B.-S., L.A.C.J.V., R.S.);Institute of Life Sciences, Scuola Superiore Sant'Anna, 56127 Pisa, Italy (H.v.V.);Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, 1090 GE Amsterdam, The Netherlands (M.A., P.v.T.);Center for Plant Cell Biology, Botany, and Plant Sciences, University of California, Riverside, California 92521 (T.G., J.B.-S.);Department of Plant Physiology, Bayreuth University, 95447 Bayreuth, Germany (A.M.); andBiosystematics Group, Wageningen University, 6708 PB Wageningen, The Netherlands (M.E.S.) r.sasidharan@uu.nl.

Abstract

Climate change has increased the frequency and severity of flooding events, with significant negative impact on agricultural productivity. These events often submerge plant aerial organs and roots, limiting growth and survival due to a severe reduction in light reactions and gas exchange necessary for photosynthesis and respiration, respectively. To distinguish molecular responses to the compound stress imposed by submergence, we investigated transcriptomic adjustments to darkness in air and under submerged conditions using eight Arabidopsis (Arabidopsis thaliana) accessions differing significantly in sensitivity to submergence. Evaluation of root and rosette transcriptomes revealed an early transcriptional and posttranscriptional response signature that was conserved primarily across genotypes, although flooding susceptibility-associated and genotype-specific responses also were uncovered. Posttranscriptional regulation encompassed darkness- and submergence-induced alternative splicing of transcripts from pathways involved in the alternative mobilization of energy reserves. The organ-specific transcriptome adjustments reflected the distinct physiological status of roots and shoots. Root-specific transcriptome changes included marked up-regulation of chloroplast-encoded photosynthesis and redox-related genes, whereas those of the rosette were related to the regulation of development and growth processes. We identified a novel set of tolerance genes, recognized mainly by quantitative differences. These included a transcriptome signature of more pronounced gluconeogenesis in tolerant accessions, a response that included stress-induced alternative splicing. This study provides organ-specific molecular resolution of genetic variation in submergence responses involving interactions between darkness and low-oxygen constraints of flooding stress and demonstrates that early transcriptome plasticity, including alternative splicing, is associated with the ability to cope with a compound environmental stress.

PMID:
27208254
PMCID:
PMC5047075
DOI:
10.1104/pp.16.00472
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center