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Biochem J. 2016 Jul 15;473(14):2131-9. doi: 10.1042/BCJ20160177. Epub 2016 May 17.

miR-30a can inhibit DNA replication by targeting RPA1 thus slowing cancer cell proliferation.

Author information

1
Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China Life Sciences School of Nanjing University, Nanjing, JS 210093, China Biochemical Department of Purdue University, West Lafayette, IN 47906, U.S.A. sokuren@163.com.
2
Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China.
3
Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China Life Sciences School of Nanjing University, Nanjing, JS 210093, China.
4
Plant Protecting School of Nanjing Agricultural University, Nanjing, JS 210093, China.

Abstract

Cell proliferation was inhibited following forced over-expression of miR-30a in the ovary cancer cell line A2780DX5 and the gastric cancer cell line SGC7901R. Interestingly, miR-30a targets the DNA replication protein RPA1, hinders the replication of DNA and induces DNA fragmentation. Furthermore, ataxia telangiectasia mutated (ATM) and checkpoint kinase 2 (CHK2) were phosphorylated after DNA damage, which induced p53 expression, thus triggering the S-phase checkpoint, arresting cell cycle progression and ultimately initiating cancer cell apoptosis. Therefore, forced miR-30a over-expression in cancer cells can be a potential way to inhibit tumour development.

KEYWORDS:

DNA replication; RPA1; S-phase checkpoint; cell cycle arrest; miR-30a

PMID:
27208176
DOI:
10.1042/BCJ20160177
[Indexed for MEDLINE]

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