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J Fluoresc. 2016 Jul;26(4):1367-72. doi: 10.1007/s10895-016-1826-z. Epub 2016 May 20.

A Turn on ESIPT Probe for Rapid and Ratiometric Fluorogenic Detection of Hg(2+) and its Application in Live-Cell Imaging.

Zhang D1,2,3, Liu J4,5,6, Yin H1,2,3, Wang H1,2,3, Li S1,2,3, Wang M1,2,3, Li M1,2,3, Zhou L1,2,3, Zhang J7,8,9.

Author information

1
Institute of Quality Standards and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
2
Henan Cereal Quality and Safety Testing Key Laboratory, Zhengzhou, 450002, China.
3
Laboratory of Quality & Safety Risk Assessment for Agro-Products (Zhengzhou), Ministry of Agriculture, Zhengzhou, 450002, China.
4
Institute of Quality Standards and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China. ljha3100@163.com.
5
Henan Cereal Quality and Safety Testing Key Laboratory, Zhengzhou, 450002, China. ljha3100@163.com.
6
Laboratory of Quality & Safety Risk Assessment for Agro-Products (Zhengzhou), Ministry of Agriculture, Zhengzhou, 450002, China. ljha3100@163.com.
7
Institute of Quality Standards and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China. zhangjff@sina.com.
8
Henan Cereal Quality and Safety Testing Key Laboratory, Zhengzhou, 450002, China. zhangjff@sina.com.
9
Laboratory of Quality & Safety Risk Assessment for Agro-Products (Zhengzhou), Ministry of Agriculture, Zhengzhou, 450002, China. zhangjff@sina.com.

Abstract

A probe based on 2-(2'-hydroxyphenyl) benzothiazole (HBT) and thiophosphate has been synthesized and used for the ratiometric detection of Hg(2+). The probe was designed in such a way that the excited state intramolecular proton transfer (ESIPT) of the HBT moiety get blocked. The probe exhibited a strong fluorescence enhancement upon addition of Hg(2+) while showing almost no response to other cations in CH3CN/HEPES buffer solution. The probe exhibited fast selectivity towards Hg(2+) and could be completed in 1 min. Fluorescence imaging experiments of Hg(2+) in living TE-1 cells demonstrated its value of practical applications in biological systems.

KEYWORDS:

Deprotection; ESIPT; Fluorescence; Hg2+; Thiophosphate

PMID:
27207569
DOI:
10.1007/s10895-016-1826-z
[Indexed for MEDLINE]

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